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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was found to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential function in promoting cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two further splice variants had been noted but in-depth nucleotide sequence details is not really but accessible.

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Blocking peptides are peptides that bind precisely to the goal antibody and block antibody binding. These peptide commonly includes the epitope regarded via the antibody.

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This guide outlines additional feasible optimization of cellular and immunoassay check here parameters to make certain the best possible success are...

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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the extremely conserved cyclin family members, whose members are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show unique expression and degradation styles which contribute into the temporal coordination of every mitotic party. Cyclin E kinds a posh with and features as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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